Reporter

Part:BBa_K2095003:Design

Designed by: Liu iGEM 2016   Group: iGEM16_Linkoping_Sweden   (2016-10-10)


mRFP1 regulated by pLIP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 866
    Illegal AgeI site found at 978
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a truncated LIP promoter in order to optimize the promoters gene activating properties.


Source

Truncated LIP promoter (BBa_K2095000) source: Dunaliella sp, RFP (BBa_E1010) source: Discosoma striata, RBCS2 terminator (BBa_K2095002) source: pOpt_mVenus_Hyg expression plasmid.

References